DNA isolation / purification Bacteria

A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. A quantitative, real-time PCR reaction typically includes all of that plus a probe that can be detected fluorescently as the reaction runs, with no gel required. for detection. However, non-specific product amplification and primer-dimer formation during set-up are major causes of PCR failure. Nevertheless, high-quality DNA polymerase and optimize reaction buffers will certainly lead to a successful PCR reaction.

Get tips on using TriPure Isolation Reagent to perform RNA isolation / purification Cells - primary human pulmonary artery endothelial cells

Get tips on using EZ-10 Spin Column PCR Product Purification kit to perform DNA gel extraction / PCR product purification Product size > 15Kb

Get tips on using HiTrap Q FF anion exchange chromatography column to perform Protein expression and purification Bacteria - Bacillus subtilis GCSF

Get tips on using TRIzol™ Plus RNA Purification Kit to perform RNA isolation / purification Cells - primary rabbit aortic endothelial cells

Get tips on using PureLink™ FFPE RNA Isolation Kit to perform RNA isolation / purification Tissue - Rat Kidney

Get tips on using High Pure FFPET RNA Isolation Kit to perform RNA isolation / purification Tissue - Rat Kidney

Get tips on using PureLink™ FFPE RNA Isolation Kit to perform RNA isolation / purification Tissue - Human Kidney

Get tips on using High Pure FFPET RNA Isolation Kit to perform RNA isolation / purification Tissue - Human Kidney

Get tips on using High Pure FFPET RNA Isolation Kit to perform RNA isolation / purification Tissue - Human Breast