siRNA / miRNA gene silencing Human MDA-MB-453

Get tips on using ROS-ID® Total ROS/Superoxide detection kit to perform ROS assay cell type - MDA-MB-231

Get tips on using anti-p62 Protein, C-Terminal Specific Polyclonal Antibody to perform Autophagy assay cell type - MDA-MB-231

Get tips on using SLC20A2 Human Gene Knockout Kit to perform CRISPR Human - Repression SLC20A2

Get tips on using FOXA2 Human Gene Knockout Kit to perform CRISPR Human - Repression FOXA2

Get tips on using Glut1 siRNA and shRNA Plasmids (h) to perform siRNA / RNAi /miRNA transfection Human Cells - HT-1376 GLUT1

Get tips on using CD74 siRNA and shRNA Plasmids (h) to perform siRNA / RNAi /miRNA transfection Human Cells - HT-1376 CD74

Get tips on using jetPEI® DNA transfection, HTS application to perform DNA transfection Mammalian cells - Immortalized cell lines MDA-MB-231

Get tips on using Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham to perform Mammalian cell culture media MDA-MB-231

Get tips on using Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham to perform Mammalian cell culture media MDA-MB-468

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase