siRNA / miRNA gene silencing Human THP-1

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Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

Cellular assays Reporter gene assay luciferase HEK 293 human embryonic kidney cells

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

Cellular assays Reporter gene assay luciferase SW480 human colon cancer cell line

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

Cellular assays Reporter gene assay β-galactosidase substrates human knee bone tissue

Get tips on using pSUPER.retro.neo+gfp vector- Syn G (exon 3) siRNA to perform shRNA gene silencing Mouse - RGC-5 Syn G (Exon 3)

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Get tips on using Atg3 Antibody to perform Autophagy assay cell type - THP 1

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Get tips on using LC3B Antibody to perform Autophagy assay cell type - THP 1

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Get tips on using LC3B Antibody to perform Autophagy assay cell type - THP 1

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Get tips on using Monoclonal Mouse Anti-Human Ki-67 Antigen (Dako Omnis) Clone MIB-1 to perform Immunohistochemistry Human - Ki-67

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Get tips on using RIPA Buffer to perform Protein isolation Mammalian cells - THP-1

Products Sigma-Aldrich RIPA Buffer

Get tips on using Trichloroacetic acid to perform Protein isolation Mammalian cells - THP-1

Products Sigma-Aldrich Trichloroacetic acid

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