RNA isolation / purification Cells Cancer cell lines Leukemia cancer cell lines

Get tips on using Direct-zol RNA Kits to perform RNA isolation / purification Cells - immortalized VCaP

Get tips on using Direct-zol RNA Kits to perform RNA isolation / purification Cells - immortalized LNCaP

Get tips on using Absolutely RNA Microprep Kit to perform RNA isolation / purification Cells - immortalized H1299

Get tips on using Direct-zol RNA Kits to perform RNA isolation / purification Cells - immortalized DuCaP

Get tips on using Direct-zol RNA Kits to perform RNA isolation / purification Cells - immortalized DU145

Get tips on using Direct-zol RNA Kits to perform RNA isolation / purification Cells - immortalized DH82

Get tips on using EpiTect Bisulfite Kit to perform DNA methylation profiling Whole genome profiling - OVCAR-3 human ovarian cancer

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

Get tips on using RNAsimple Total RNA Kit to perform RNA isolation / purification Cells - immortalized MA-104

DNA isolation and purification is the first critical step in sample preparation that helps ensure optimal performance of downstream assays like PCR, microarrays, and sequencing. Failure in yielding high-quality DNA would be the major reason that DNA doesn't work for the downstream application. To circumvent this, one should follow the recommended storage conditions to minimize DNA degradation by nucleases and shouldn't overload the purification system.