crispr-human-deletion-f11r

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Get tips on using Monoclonal Mouse Anti-Human E-Cadherin (Dako Omnis) Clone NCH-38 to perform Immunohistochemistry Human - E-Cadherin

Products Agilent Technologies Monoclonal Mouse Anti-Human E-Cadherin (Dako Omnis) Clone NCH-38

Get tips on using Monoclonal Mouse Anti-Human Ki-67 Antigen (Concentrate) Clone MIB-1 to perform Immunohistochemistry Human - Ki-67

Products Agilent Technologies Monoclonal Mouse Anti-Human Ki-67 Antigen (Concentrate) Clone MIB-1

Get tips on using GeneChip® Human Genome U133 Plus 2.0 Array to perform Microarray Gene expression arrays - Rhesus monkey brain tissue Biotin

Products Thermo Fisher Scientific GeneChip® Human Genome U133 Plus 2.0 Array

Acid phosphatase detection heavily relies on determining the concentration of tartrate-resistant acid phosphatase (TRAP) in the sample. Hence, sample preparation is very crucial and it should be done strictly as per kit manufacturer instructions to avoid any inconsistency and poor sensitivity

Cellular assays Acid phosphatase assay cell type human periodontal ligament cells

Get tips on using Anti-Human FMC7 FITC/CD23 PE/CD19 PerCP-Cy™5.5 to perform Flow cytometry Anti-bodies Human - CD23

Products BD Biosciences Anti-Human FMC7 FITC/CD23 PE/CD19 PerCP-Cy™5.5

RNA siRNA / miRNA gene silencing Human U87MG HES6

RNA siRNA / miRNA gene silencing Human U87MG PLK1

RNA RNA isolation / purification Tissue Human FFPE tissue

Get tips on using CD171 (L1CAM) Antibody, anti-human, PE-Vio® 770, REAfinity™ to perform Flow cytometry Anti-bodies Human - CD171/L1CAM

Products Miltenyibiotec CD171 (L1CAM) Antibody, anti-human, PE-Vio® 770, REAfinity™

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

DNA Site Directed Mutagenesis (SDM) Human Point mutation HeLa CLN5

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