Immunohistochemistry CD3 Mouse Human

Get tips on using Human Endoglin/CD105 PE-conjugated Antibody to perform Flow cytometry Anti-bodies Human - CD105

Get tips on using PerCP/Cyanine5.5 anti-human CD49d Antibody to perform Flow cytometry Anti-bodies Human - CD49d

Get tips on using PerCP/Cyanine5.5 anti-human CD4 Antibody to perform Flow cytometry Anti-bodies Human - CD4

Get tips on using APC anti-human CD90 (Thy1) Antibody to perform Flow cytometry Anti-bodies Human - CD90

Get tips on using Human IL-6R alpha Antibody to perform Flow cytometry Anti-bodies Human - CD126/IL-6Ralpha

Get tips on using Accell Human CD44 (960) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - HaCaT CD44

Get tips on using EasySep™ Human CD33 Positive Selection Kit II to perform Cell Isolation Monocyte

Get tips on using PE anti-human CD135 (Flt-3/Flk-2) Antibody to perform Flow cytometry Anti-bodies Human - CD135

Get tips on using EasySep™ Human Cord Blood CD34 Positive Selection Kit II to perform Cell Isolation CD34+ cells

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.