DNA microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.
DNA microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.
Get tips on using Cell Lysis Buffer (10X) to perform Protein isolation Mammalian cells - THP-1
Get tips on using Atg12 (D88H11) Rabbit mAb to perform Autophagy assay cell type - THP 1
Get tips on using Atg7 (D12B11) Rabbit mAb to perform Autophagy assay cell type - THP 1
Get tips on using Atg5 (D5F5U) Rabbit mAb to perform Autophagy assay cell type - THP 1
Get tips on using Cell Death Detection ELISAPLUS to perform Apoptosis assay cell type - THP-1
Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - immortalized THP-1
DNA microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.
Get tips on using HyClone RPMI 1640 media: Liquid to perform Mammalian cell culture media THP-1
Fill out your contact details and receive price quotes in your Inbox
Outsource experiment