siRNA / miRNA gene silencing Mouse B16-BL6

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Get tips on using Ad-Sal-shRNA to perform shRNA gene silencing Rat - WKY Salusin-β

Products Genomeditech Co. Ad-Sal-shRNA

DNA microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.

DNA Microarray Comperative genomic hybridization Mouse iPSC

Get tips on using miRNA Complete Labeling and Hyb Kit to perform Microarray RNA amplification & Labeling - Human endometrial stromal cells Cyanine 3-pCp

Products Agilent Technologies miRNA Complete Labeling and Hyb Kit

Get tips on using Mouse Reg1 Antibody to perform Immunohistochemistry Mouse - Reg1

Products R&D system, Minneapolis, MN, USA Mouse Reg1 Antibody

Get tips on using Mouse Prolactin ELISA to perform ELISA Mouse - PRL

Products Raybiotech Mouse Prolactin ELISA

Get tips on using Mouse Adiponectin ELISA to perform ELISA Mouse - Adiponectin

Products Merck Millipore Mouse Adiponectin ELISA

Get tips on using Purified Mouse Anti-Nucleoporin p62 to perform Western blot p62/SQSTM1 - Mouse IgG2b Human -NA-

Products BD Transduction Laboratories Purified Mouse Anti-Nucleoporin p62

Get tips on using GeneSilencer® Transfection Reagent to perform siRNA / RNAi /miRNA transfection Rat - C6 Cationic lipid based

Products Genlantis GeneSilencer® Transfection Reagent

Get tips on using Rock-2 siRNA and shRNA Plasmids (h) to perform RNA sequencing Human - HT-1376 (urinary bladder cell line)

Products Santa Cruz Biotechnology Rock-2 siRNA and shRNA Plasmids (h)

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

Proteins ELISA Mouse Adiponectin

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