siRNA / miRNA gene silencing Mouse NIH-3T3

Get tips on using SurePrint Human miRNA Microarrays to perform Microarray Human - Endometrial stromal cells miRNA-expression array (labelled)

Get tips on using mirVana® miRNA mimic to perform RNA isolation / purification Cells - primary human endothelial cells

Get tips on using NucleoSpin® miRNA to perform RNA isolation / purification Tissue - Human Brain

Get tips on using NucleoSpin® miRNA to perform RNA isolation / purification Tissue - Rat Artery / Aorta

Get tips on using Alexa Fluor® 700 Mouse Anti-Mouse NK1.1 to perform Flow cytometry Anti-bodies Mouse - NK1.1

Get tips on using NucleoSpin® miRNA to perform RNA isolation / purification Cells - primary canine aortic endothelial cells

Get tips on using NucleoSpin® miRNA to perform RNA isolation / purification Cells - primary human mesenchymal stem cells

DNA-protein interactions are studied by using ChIP. The basic steps in this technique are crosslinking, sonication, immunoprecipitation, and analysis of the immunoprecipitated DNA. During ChIP, if chromatin is under-fragmented or fragments are too large which can lead to the increased background and lower resolution. Shorter cross-linking times (5-10 min) and/or lower formaldehyde concentrations (<1%) may improve shearing efficiency. If Chromatin is over-fragmented, then optimize shearing conditions for each cell type to improve ChIP efficiency. Over-sonication of chromatin may disrupt chromatin integrity and denature antibody epitopes. If you do not see any product or very little product in the input PCR reactions, add 5–10 μg chromatin per IP.

Get tips on using PAXgene Tissue miRNA Kit to perform RNA isolation / purification Tissue - Rat Brain

Get tips on using miRcute miRNA Isolation Kit to perform RNA isolation / purification Cells - immortalized H1299