siRNA / miRNA gene silencing Rat Schwann cells

Get tips on using Rat Activin-A ELISA to perform ELISA Rat - Activin

Get tips on using Rat Adiponectin ELISA Kit to perform ELISA Rat - Adiponectin

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.

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Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Get tips on using Rat VEGF ELISA Kit (ab100786) to perform ELISA Rat - VEGF

Get tips on using Rat VEGF Quantikine ELISA Kit to perform ELISA Rat - VEGF

Get tips on using Rat prolactin,PRL ELISA Kit to perform ELISA Rat - PRL

Get tips on using Rat GDNF ELISA Kit (ab213901) to perform ELISA Rat - GDNF

Get tips on using Rat Fibronectin ELISA Kit (ab108850) to perform ELISA Rat - Fibronectin