DNA isolation / purification Bacteria Gram negative

Get tips on using XcelGen Plant RNA Isolation Mini Kit to perform RNA isolation / purification Plants - Seeds

Get tips on using Y-PER™ Yeast Protein Extraction Reagent to perform Protein isolation Bacteria - Escherichia coli

Get tips on using GeneJET RNA Purification Kit to perform RNA isolation / purification Cells - primary human renal artery smooth muscle cells

Get tips on using miRCURY RNA Isolation Kit to perform RNA isolation / purification Cells - immortalized SK-BR-3

Get tips on using miRCURY RNA Isolation Kit to perform RNA isolation / purification Cells - immortalized MDA-MB-231

Get tips on using miRcute miRNA Isolation Kit to perform RNA isolation / purification Cells - immortalized MDA-MB-157

A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. Multiplexing such a reaction amplifies the design challenges where one target requires 3 primers, which should be exclusively bound nowhere in the template DNA or to each other. Similarly, two targets require 6, three require 9, and so on. Each amplicon needs to be either a different size (for gels) or labeled with a different fluorescent tag that is spectrally distinct from the others in the reaction. Further complicating this, different targets in the reaction can compete with each other for resources and causes more challenges in the detection of amplicons. However, with proper primer designing, their validation, optimize quality and concentration of the enzyme and buffers certainly lead to a successful multiplex PCR reaction.

Get tips on using SV Total RNA Isolation System to perform RNA isolation / purification Cells - immortalized MCF-7

Get tips on using SV Total RNA Isolation System to perform RNA isolation / purification Tissue - Mouse Adrenal glands

Get tips on using High Pure miRNA Isolation Kit to perform RNA isolation / purification Tissue - Human Bone marrow