TissueFAxs 53BP1 [H-300]

Get tips on using Human Adiponectin ELISA Kit (ab108786) to perform ELISA Human - Adiponectin

Get tips on using Human Adiponectin/Acrp30 DuoSet ELISA to perform ELISA Human - Adiponectin

Get tips on using Human Synoviocyte Growth Medium to perform Mammalian cell culture media HFLS

Get tips on using Hs_PLK1_7 FlexiTube siRNA to perform siRNA / miRNA gene silencing Human - U87MG PLK1

Get tips on using Hs_GLI1_1 FlexiTube siRNA to perform siRNA / miRNA gene silencing Human - DAOY GLI1

Get tips on using Hs_PLK1_7 FlexiTube siRNA to perform siRNA / miRNA gene silencing Human - A172 PLK1

Get tips on using jetPEI®-HUVEC to perform DNA transfection Mammalian cells - Primary cells HUVEC

Get tips on using HuR siRNA to perform siRNA / miRNA gene silencing Rat - IEC-6 HuR Lipid

DNA microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.

DNA microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.