DNA methylation profiling Gene specific profiling Caco-1

Get tips on using 100bp DNA Step Ladder to perform DNA Ladder 100 bp

Get tips on using 100 bp DNA Ladder to perform DNA Ladder 100 bp

Get tips on using 123 bp DNA Ladder to perform DNA Ladder 123 bp

Get tips on using TIANamp Genomic DNA Kit to perform DNA isolation / purification Cells - Immortalized cell lines HEK 293T

Get tips on using Ni-NTA Magnetic Agarose Beads (6 x 1 ml) to perform Protein tag Purification of His-tagged proteins

Though DNA quantification is but one small step in the multifaceted DNA sample preparation workflow, it can have large implications on the performance and validity of conclusions drawn from downstream assays. Major challenges include accuracy, precision, reproducibility, and detection of present contamination. Among UV spectrophotometry, fluorescence and real-time PCR based methods, the quantification method should be chosen based on the requirement of the downstream assay.

Cell Invasion or Cell Migration assays are technically challenging to set up as the gradient between the two compartments equilibrates in time during the assay. It is also problematic to view cells and for cells to migrate through a non-physiologic polycarbonate or polypropylene filter. Care must be taken while loading the well with cells to form a single cell suspension. Precaution must be taken while trypsinization (under-trypsinization can lead to cell clumping while over-trypsinization could strip off adhesion molecules necessary for migration). This leads to difficulty in getting significant results, when only small numbers of cells cross the filter or when the distribution and/or staining of the cells is uneven.

Cell Invasion or Cell Migration assays are technically challenging to set up as the gradient between the two compartments equilibrates in time during the assay. It is also problematic to view cells and for cells to migrate through a non-physiologic polycarbonate or polypropylene filter. Care must be taken while loading the well with cells to form a single cell suspension. Precaution must be taken while trypsinization (under-trypsinization can lead to cell clumping while over-trypsinization could strip off adhesion molecules necessary for migration). This leads to difficulty in getting significant results, when only small numbers of cells cross the filter or when the distribution and/or staining of the cells is uneven.

Get tips on using TIANamp Genomic DNA Kit to perform DNA isolation / purification Cells - Primary cells Mouse embryonic fibroblast (MEF)

Get tips on using s4215 to perform siRNA / miRNA gene silencing Human - PANC-1 DNMT1/3b