Cell migration / Invasion cell type - PANC-1

Cell Invasion or Cell Migration assays are technically challenging to set up as the gradient between the two compartments equilibrates in time during the assay. It is also problematic to view cells and for cells to migrate through a non-physiologic polycarbonate or polypropylene filter. Care must be taken while loading the well with cells to form a single cell suspension. Precaution must be taken while trypsinization (under-trypsinization can lead to cell clumping while over-trypsinization could strip off adhesion molecules necessary for migration). This leads to difficulty in getting significant results, when only small numbers of cells cross the filter or when the distribution and/or staining of the cells is uneven.

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Found 3 matching solutions for this experiment

Upstream tips
Seed 20,000 cells per well
Protocol tips
Incubate for 2-24 hours in a cell culture incubator.
Downstream tips
Measure the OD 560nm in a
plate reader.
Upstream tips
Seed 2.0 × 10^5 cells.
Protocol tips
Cover plate and incubate for 48 hours at 37°C in a CO2 incubator (4- 6% CO2).
Protocol tips
Assemble chamber and incubate at 37C in a CO2 incubator for 4-48 hours
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