ChIP acH3 Bovine Bovine

DNA-protein interactions are studied by using ChIP. The basic steps in this technique are crosslinking, sonication, immunoprecipitation, and analysis of the immunoprecipitated DNA. During ChIP, if chromatin is under-fragmented or fragments are too large which can lead to the increased background and lower resolution. Shorter cross-linking times (5-10 min) and/or lower formaldehyde concentrations (<1%) may improve shearing efficiency. If Chromatin is over-fragmented, then optimize shearing conditions for each cell type to improve ChIP efficiency. Over-sonication of chromatin may disrupt chromatin integrity and denature antibody epitopes. If you do not see any product or very little product in the input PCR reactions, add 5–10 μg chromatin per IP.

Get tips on using EpiTect ChIP qPCR Assays to perform ChIP Rat - Brain

Get tips on using EpiTect ChIP OneDay Kit to perform ChIP Mouse - MIN6

Get tips on using EpiTect ChIP OneDay Kit to perform ChIP Human - AGS

Get tips on using Re-ChIP-IT® to perform ChIP Human - LCL

Get tips on using EpiTect ChIP qPCR Assays to perform ChIP Mouse - NIH3T3

Get tips on using Anti-Rad51 antibody - ChIP Grade (ab176458) to perform ChIP Anti-bodies RAD51

Get tips on using Anti-Mre11 antibody - ChIP Grade (ab12159) to perform ChIP Anti-bodies MRE11

Get tips on using Anti-CTCF antibody - ChIP Grade (ab70303) to perform ChIP Anti-bodies CTCF

Get tips on using Anti-Sall4 antibody - ChIP Grade (ab29112) to perform ChIP Anti-bodies Sall4