Site Directed Mutagenesis (SDM) Mouse Point mutation Neuro 2a

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Get tips on using Purified Mouse Anti-β-Catenin Clone 14/Beta-Catenin (RUO) to perform Immunohistochemistry Human - β-catenin

Products BD Biosciences Purified Mouse Anti-β-Catenin Clone 14/Beta-Catenin (RUO)

RNA RNA isolation / purification Cells primary mouse morula cells

Get tips on using Mouse Monoclonal Antibody to Lamin A/C Cat# MCA-4C4 to perform Western blotting Lamin A/C

Products EnCor Biotechnology Inc. Mouse Monoclonal Antibody to Lamin A/C Cat# MCA-4C4

Get tips on using Mouse/Rat/Porcine/Canine TGF-beta 1 Quantikine ELISA Kit to perform ELISA Rat - TGF-beta 1

Products R&D Systems Mouse/Rat/Porcine/Canine TGF-beta 1 Quantikine ELISA Kit

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Cellular assays Autophagy assay cell type Mouse aortic endothelial cells (MAECs)

Cell culture media 3D Cell Culture Media Mouse gastric cancer organoids

Get tips on using SurePrint G3 Mouse Exon 4x180K Microarray Kit (165,984 Exon probes) to perform Microarray Mice - Cochlea Expression array (labelled)

Products Agilent Technologies SurePrint G3 Mouse Exon 4x180K Microarray Kit (165,984 Exon probes)

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.

RNA RNA isolation / purification Cells primary mouse pulmonary artery smooth muscle cells

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that no responses other than those related to the signaling pathway of interest. This can be achieved by selecting a highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzyme such as luciferase.

Cellular assays Reporter gene assay β-galactosidase substrates mouse mesenchymal stem cells

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

Cellular assays Reporter gene assay β-galactosidase substrates mouse pancreatic stellate cells

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