crispr-mouse-activation-3t3-l1-c-ebp

Get tips on using pMXs-IRES-Bsd Retroviral Expression Vector to perform CRISPR Human - Repression DDX3Y

Get tips on using peSpCas9(1.1)-2×sgRNA (empty, donor) to perform CRISPR Human - Repression SLC7A11

Get tips on using pMXs-IRES-Bsd Retroviral Expression Vector to perform CRISPR Human - Repression DDX3X

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Get tips on using Monoclonal Mouse Anti-Human Cytokeratin 7 (Dako Omnis) Clone OV-TL 12/30 to perform Immunohistochemistry Human - CK7

Get tips on using DIA-310: Anti-CD31 (Ms) from Rat (Clone: SZ31) for mouse FFPE tissue to perform Immunohistochemistry CD31 - Rabbit Rat -NA-

Get tips on using pX330-U6-Chimeric_BB-CBh-hSpCas9 to perform CRISPR Human - Repression PrPC

Get tips on using pX330-U6-Chimeric_BB-CBh-hSpCas9 to perform CRISPR Human - Repression EZH2

Get tips on using pX330-U6-Chimeric_BB-CBh-hSpCas9 to perform CRISPR Human - Repression EZH2

Get tips on using pX330-U6-Chimeric_BB-CBh-hSpCas9 to perform CRISPR Human - Repression HBV