DNA Damage Assay Human bronchial epithelial cells (hBE)

Get tips on using In Situ Cell Death Detection Kit, TMR red to perform TUNEL assay cell type - A127, U87MG, U251MG, T98G human glioblastoma cells

Get tips on using ROS-Glo™ H2O2 Assay to perform ROS assay cell type - HEK293

Get tips on using ROS-Glo™ H2O2 Assay to perform ROS assay cell type - HeLa

Get tips on using EZ DNA Methylation kit to perform DNA methylation profiling Gene specific profiling - Hypothalamus mouse tissue MeCP2

Get tips on using Cell Comb™ Scratch Assay to perform Wound healing assay cell type - mouse MS1

Get tips on using EZ DNA Methylation-Gold Kit to perform DNA methylation profiling Gene specific profiling - HepG2 Constitutive Androstane Receptor (CAR)

Get tips on using MammoCult™ Human Medium Kit to perform 3D Cell Culture Media Human primary breast ephitelial cells-Mammospheres

Get tips on using Acid phosphatase Assay Kit to perform Acid phosphatase assay cell type - OV2008

A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. A quantitative, real-time PCR reaction typically includes all of that plus a probe that can be detected fluorescently as the reaction runs, with no gel required. for detection. However, non-specific product amplification and primer-dimer formation during set-up are major causes of PCR failure. Nevertheless, high-quality DNA polymerase and optimize reaction buffers will certainly lead to a successful PCR reaction.

A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. A quantitative, real-time PCR reaction typically includes all of that plus a probe that can be detected fluorescently as the reaction runs, with no gel required. for detection. However, non-specific product amplification and primer-dimer formation during set-up are major causes of PCR failure. Nevertheless, high-quality DNA polymerase and optimize reaction buffers will certainly lead to a successful PCR reaction.