siRNA / RNAi /miRNA transfection Rat H9c2

Get tips on using Mammalian beta-Galactosidase Assay Kit to perform Reporter gene assay β-galactosidase substrates - H9C2

Get tips on using Live/Dead cell Staining Kit II to perform Live / Dead assay mammalian cells - H9C2

Get tips on using SiRNA silencing human Eph receptor B4, Id: s243 to perform siRNA / miRNA gene silencing Human - HNSCC cell line Eph receptor B4 Polymer / Lipid

Get tips on using SiRNA silencing human Eph receptor B4, Id: 533 to perform siRNA / miRNA gene silencing Human - HNSCC cell line Eph receptor B4 Polymer / Lipid

Get tips on using Whole Rat Genome Microarray Kit, 4x44K to perform Microarray Gene expression arrays - Rat chorid plexus Cyanine 3

Get tips on using Rat GE 4x44K v3 Microarray Kit to perform Microarray Gene expression arrays - Rat pancreas tissue Cyanine 3 & cyanine 5

Get tips on using Rat GE 4x44K v3 Microarray Kit to perform Microarray Gene expression arrays - Rat cholangio carcinoma cyanine 3 & cyanine 5

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.