RNA sequencing

Get tips on using RNeasy Micro Kit to perform RNA isolation / purification Tissue - Rat Pancreas

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Tissue - Rat Ovaries

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Tissue - Rat Muscles

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Tissue - Rat Jejunum

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Tissue - Rat ileum

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Tissue - Rat Duodenum

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Tissue - Rat Decidua

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Tissue - Rat Bone

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - immortalized Raji

The RNA interference (RNAi) is used to inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Two types of RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA) play a central role in RNAi. Few points have to considered to increase the transfection efficiency of siRNA. Always use healthy, actively dividing cells to maximize transfection efficiency. The confluency of cells should be between 50-70%. Always use the most appropriate siRNA concentration to avoid off-target effects and unwanted toxic side effects. Positive and negative controls should be used for each and every experiment to determine transfection efficiency.