siRNA / miRNA gene silencing Human BCBL-1

A key signature for necrotic cells is the permeabilization of the plasma membrane. Necrosis can be quantified by several cellular and biochemical assays. When studied minutely, it reveals the difficulty in confirmation in secondary induction of necrosis in apoptotic cells. Apoptotic cells are being analyzed to shift to necrotic status owing to membrane permeability at later stages, and thus, discrimination of two cell death becomes critical. Therefore, it is crucial to use a necrosis detection kit or a defined procedure to analyze this unprogrammed form of death in response to immense chemical and physical insults.

Get tips on using GDNF RECEPTOR ALPHA 1 to perform Immunohistochemistry Mouse - GFRA1

Get tips on using CD31/PECAM-1 Antibody to perform Immunohistochemistry Mouse - CD31

Get tips on using PAXgene Tissue miRNA Kit to perform RNA isolation / purification Tissue - Rat Brain

Get tips on using miRcute miRNA Isolation Kit to perform RNA isolation / purification Cells - immortalized H1299

Transfection is a powerful technique that enables the study of the function of genes and gene products in cells. Based on the nature of experiments, we may need a stable DNA transfection in cells for persistent gain-of-function or loss-of-function of the target gene. For stable transfection, integration of a DNA vector into the chromosome is crucial which requires selective screening and clonal isolation. By carefully selecting a viral delivery system and related reagents we can ensure safe and highly-efficient delivery of expression constructs for high-level constitutive or inducible expression in any mammalian cell type.

Get tips on using SCGB1A1 antibody (Secretoglobin, Family 1A, Member 1 (Uteroglobin)) (Middle Region) to perform Immunohistochemistry Human - SCGB1A1 /CC10

Get tips on using Human PAI1 ELISA Kit (SERPINE1) (ab184863) to perform ELISA Human - Serpin E1/PAI-1

Get tips on using 1 kb DNA ladder-TLAD-1K to perform DNA Ladder 1 kb

Get tips on using ZO-1 Monoclonal Antibody (ZO1-1A12) to perform Western blotting ZO-1