Site Directed Mutagenesis (SDM) Human Point mutation THP-1

Get tips on using ON-TARGETplus Human AQP5 (362) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - K562 AQP5

Get tips on using ON-TARGETplus Human MET (4233) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - H1299 MET

Get tips on using ON-TARGETplus Human ITGB4 (3691) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - DU145 ITGB4

Get tips on using ON-TARGETplus Human PLAU (5328) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - DU145 PLAU

Get tips on using ON-TARGETplus Human EPCAM (4072) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - DU145 EPCAM

Get tips on using ON-TARGETplus Human IRF3 (3661) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - A549 IRF3

Get tips on using ON-TARGETplus Human MET (4233) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - A431 MET

Get tips on using ON-TARGETplus Human FURIN (5045) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - A253 Furin

Flow cytometry is an immunophenotyping technique whereby sing cell suspensions are stained for either cell surface markers or intracellular proteins by fluorescently-labelled antibodies and analyzed with a flow cytometer, where fluorescently-labelled molecules are excited by the laser to emit light at varying wavelengths, which is then detected by the instrument. There are several key criteria which are required to be kept in mind while designing a flow experiment- 1. Antibody titration (optimal dilution of antibodies should be calculated in order to avoid over- or under- saturated signals for proper detection of surface and intracellular markers), 2. Precision (3 or more replicates of the sample should be used per experiment), 3. Specificity (proper isotype controls should be included in the experiment), 4. Day-to-day variability (experiments should be repeated 3 or more times to ensure consistency and avoid variability due to flow cytometer settings), 5. Antibody interaction (Fluorescence minus one or FMO should be used, which is the comparison of signals from panel minus one antibody vs. the full panel), and 6. Antibody stability (fluorescently-labelled antibodies should be stored at 4C).

Get tips on using ON-TARGETplus Human PAK1 (5058) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - Caco-2 PAK1