siRNA / miRNA gene silencing Rat Brain endothelial cells HIF-1α

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human dermal microvascular endothelial cells

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human coronary artery endothelial cells

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary bovine pulmonary artery endothelial cells

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary bovine coronary artery endothelial cells

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary human aortic endothelial cells

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary canine aortic endothelial cells

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ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

Get tips on using NucleoSpin® miRNA to perform RNA isolation / purification Tissue - Rat Artery / Aorta

Get tips on using Rat GE 4x44K v3 Microarray Kit to perform Microarray Gene expression arrays - Rat pancreas tissue Cyanine 3 & cyanine 5