Isolating DNA from tissues and paraffin-embedded tissue samples can be challenging as double-stranded DNA is physically fragile and highly susceptible to exo- and endonucleases. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in the presence of DNAse inhibitors. Further, extracting DNA from the nucleus need specific methods by combining physical, mechanical and chemical lysis approaches,
Get tips on using RiboPure™ RNA Purification Kit, bacteria to perform
Get tips on using RiboPure™ RNA Purification Kit, bacteria to perform
Get tips on using Lipofectamine® 2000 Transfection Reagent to perform DNA transfection Mammalian cells - Immortalized cell lines SKOV-3
Get tips on using FuGENE® HD Transfection Reagent to perform DNA transfection Mammalian cells - Immortalized cell lines SKOV-3
Get tips on using TransIT®-LT1 Transfection Reagent to perform DNA transfection Mammalian cells - Immortalized cell lines SKOV-3
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