siRNA / miRNA gene silencing Human UCC (urothelial cancer cell lines)

Get tips on using B Cell Isolation Kit II, human to perform Cell Isolation B cell

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Get tips on using FastLane Cell cDNA Kit (50) to perform cDNA synthesis Cell lines

Get tips on using RNAprotect Cell Reagent (250 ml) to perform RNA stabilization Cell lines

Get tips on using RosetteSep™ Human T Cell Enrichment Cocktail to perform Cell Isolation Human T cells

Get tips on using MagniSort™ Human T cell Enrichment Kit to perform Cell Isolation Human T cells

Get tips on using EasySep™ Human T Cell Enrichment Kit to perform Cell Isolation Human T cells

Get tips on using EasySep™ Human T Cell Isolation Kit to perform Cell Isolation Human T cells

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.