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Get tips on using Comet Assay Kits, 96-Well to perform DNA Damage Assay MIA PaCa-2

Get tips on using Comet Assay Kits, 96-Well to perform DNA Damage Assay MDA-MB-231

Get tips on using Microbial Viability Assay Kit-WST to perform Live / Dead assay bacteria - Listeria innocua

Get tips on using Microbial Viability Assay Kit-WST to perform Live / Dead assay bacteria - Staphylococcus epidermidis

Get tips on using In Vitro Angiogenesis Assay Kit to perform Angiogenesis assay human - hiPSC-2-EC

Get tips on using In Vitro Angiogenesis Assay Kit to perform Angiogenesis assay human - hiPSC-1-EC

Get tips on using In Vitro Angiogenesis Assay Kit to perform Angiogenesis assay mouse - spleen-derived EPCs

Transfection is a powerful technique that enables the study of the function of genes and gene products in cells. Based on the nature of experiments, we may need a stable DNA transfection in cells for persistent gain-of-function or loss-of-function of the target gene. For stable transfection, integration of a DNA vector into the chromosome is crucial which requires selective screening and clonal isolation. By carefully selecting a viral delivery system and related reagents we can ensure safe and highly-efficient delivery of expression constructs for high-level constitutive or inducible expression in any mammalian cell type.

Transfection is a powerful technique that enables the study of the function of genes and gene products in cells. Based on the nature of experiments, we may need a stable DNA transfection in cells for persistent gain-of-function or loss-of-function of the target gene. For stable transfection, integration of a DNA vector into the chromosome is crucial which requires selective screening and clonal isolation. By carefully selecting a viral delivery system and related reagents we can ensure safe and highly-efficient delivery of expression constructs for high-level constitutive or inducible expression in any mammalian cell type.

A gross majority of classical apoptotic attributes can be quantitatively examined by flow cytometry, the preferred platform for rapid assessment of multiple cellular attributes at a single-cell level. However, sample preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.