Apoptosis assay cell type - Caspase 3/7

A gross majority of classical apoptotic attributes can be quantitatively examined by flow cytometry, the preferred platform for rapid assessment of multiple cellular attributes at a single-cell level. However, sample preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

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Found 2 matching solutions for this experiment

Protocol tips
Add 195 μL of 1× assay buffer and 5 μL of Annexin V-fluorescein isothiocyanate (FITC) and incubate at room temperature in the dark,

Later add 300 μL of 1× assay buffer and 10 μL of PI (20 μg/mL)
Protocol tips
Dilute the reagent
1:1000 in complete medium
(5 μM final concentration).
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