siRNA / miRNA gene silencing Human Melanoma cells (501 Mel and SK Mel 28)

Get tips on using TransMessenger Transfection Reagent (0.5 ml) to perform siRNA / RNAi /miRNA transfection Mouse - Primary cortical and hippocampal cell

A gross majority of classical apoptotic attributes can be quantitatively examined by flow cytometry, the preferred platform for rapid assessment of multiple cellular attributes at a single-cell level. However, sample preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Get tips on using Xfect™ Transfection Reagent to perform siRNA / RNAi /miRNA transfection Rat - H9c2 Cationic and neutral lipids

Get tips on using M-PER™ Mammalian Protein Extraction Reagent to perform Protein isolation Mammalian cells - SK-N-BE(2)-C

Get tips on using Attractene Transfection Reagent to perform shRNA gene silencing Human - HEK 293T CAPN5- (Calpains) cationic lipid based

Get tips on using Nucleofector™ Kits for Human T Cells to perform DNA transfection Mammalian cells - Primary cells CD8+ T cells

Get tips on using Nucleofector™ Kits for Human T Cells to perform DNA transfection Mammalian cells - Primary cells CD4+ T cells

Get tips on using Dynabeads™ Untouched™ Human T Cells Kit to perform Cell Isolation Human T cells

Get tips on using GeneChip® Human Genome U133 Plus 2.0 Array to perform Microarray Gene expression arrays - Human endometrial stromal cells Biotin

Get tips on using Live and Dead Cell Assay (Abcam) to perform Live / Dead assay mammalian cells - human Mesenchymal stem cells