Immunohistochemistry Anti-mouse IgG Donkey Mouse

In ChIP, the most vital step is the binding of an antibody and choosing the right antibody. The binding affinity of different types of immunoglobulins to protein A or G differs significantly. Henceforth, it is recommended to choose either protein A or protein G coated beads. If you do not see any product in the positive control, add 5–10 μg of chromatin and 1–5 μg of antibody to each IP reaction and incubate with antibody overnight and an additional 2 hr after adding Protein G/A beads. If no product is observed in the experimental sample, add more DNA to the PCR reaction or increase the number of amplification cycles. Furthermore, if you have any problem with antibodies, make sure to use the ChIP-validated antibody.

Get tips on using Recombinant Anti-GATA3 antibody [EPR16651] - ChIP Grade (ab199428) to perform ChIP Anti-bodies GATA3

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Get tips on using CRISP3 Polyclonal antibody to perform Immunohistochemistry Human - CRISP3

Get tips on using Villin Monoclonal antibody to perform Immunohistochemistry Human - Villin

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