siRNA / miRNA gene silencing Human Primary Endometrial Stromal Cells hsa-miR-542-3p

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Cells - primary human aortic smooth muscle cells

Get tips on using GeneArt™ Site-Directed Mutagenesis PLUS System to perform Site Directed Mutagenesis (SDM) Human - Insertion SKOV3 miR-134

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Cells - primary human keratinocytes

Get tips on using Cell Meter™ Autophagy Assay Kit *Green Fluorescence to perform Autophagy assay cell type - Mesenchymal stromal cells

Get tips on using VENTANA anti-MLH1 (M1) Mouse Monoclonal Primary Antibody to perform Immunohistochemistry Human - MLH1

Get tips on using Galacto-Star™ β-Galactosidase Reporter Gene Assay System for Mammalian Cells to perform Reporter gene assay β-galactosidase substrates - MCF-7 human breast cancer

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Cells - primary human cardiac fibroblasts

Get tips on using VENTANA anti-MSH2 (G219-1129) Mouse Monoclonal Primary Antibody to perform Immunohistochemistry Human - MSH2

Get tips on using GeneJuice® Transfection Reagent to perform DNA transfection Mammalian cells - Primary cells Human osteoblasts