siRNA / miRNA gene silencing Rat Brain endothelial cells

Get tips on using RNeasy Plus Micro Kit to perform RNA isolation / purification Cells - primary human lung microvascular endothelial cells

Get tips on using TRI Reagent® MRC to perform RNA isolation / purification Cells - primary human dermal microvascular endothelial cells

Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Cells - primary human carotid artery endothelial cells

Get tips on using RNeasy Lipid Tissue Mini Kit to perform RNA isolation / purification Cells - primary human vascular endothelial cells

Get tips on using RNeasy Lipid Tissue Mini Kit to perform RNA isolation / purification Cells - primary bovine aortic endothelial cells

Get tips on using Galacto-Star™ β-Galactosidase Reporter Gene Assay System for Mammalian Cells to perform Reporter gene assay β-galactosidase substrates - MCF-7 human breast cancer

Isolating RNA from tissues and paraffin-embedded tissue samples can be challenging due to cross-linking of biomolecules and fragmented nucleic acids. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in presence of RNAse inhibitors. The homogenization process should be carried out on dry ice to maintain the integrity of RNA

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

Get tips on using PAXgene Tissue miRNA Kit to perform RNA isolation / purification Tissue - rat heart muscle tissue

Get tips on using PureLink™ RNA Mini Kit to perform RNA isolation / purification Cells - primary human umbilical vein endothelial cells