Protein isolation Mammalian cells

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Get tips on using Mouse CRP / C Reactive Protein / PTX1 PicoKine™ ELISA Kit to perform ELISA Mouse - C-Reactive Protein/CRP

Products BosterBio Mouse CRP / C Reactive Protein / PTX1 PicoKine™ ELISA Kit

Get tips on using SV 96 Total RNA Isolation System to perform RNA isolation / purification Cells - immortalized PK-1

Products Promega SV 96 Total RNA Isolation System

Get tips on using SV 96 Total RNA Isolation System to perform RNA isolation / purification Cells - immortalized Calu-3

Products Promega SV 96 Total RNA Isolation System

Get tips on using Anti-Glial Fibrillary Acidic Protein Antibody, clone GA5 to perform Immunohistochemistry Rat - GFAP

Products Merck Millipore Anti-Glial Fibrillary Acidic Protein Antibody, clone GA5

Get tips on using Mouse Retinol Binding Protein 4 ELISA Kit (ab202404) to perform ELISA Mouse - RBP4

Products Abcam Mouse Retinol Binding Protein 4 ELISA Kit (ab202404)

I am currently using a recombinant protein which shows metal-dependent DNase activity. Is it possible to pinpoint the source of the DNase activity after protein purification? More specifically, can I ensure that the DNase activity is not because of nuclease contamination from the E.coli that might have persisted and passed with the protein of interest during purification?

Discussions Is a bacterial nuclease contamination possible during protein purification?

Get tips on using miRCURY RNA Isolation Kit to perform RNA isolation / purification Cells - immortalized SKBR3, MDA-MB231 and MCF7

Products Exiqon miRCURY RNA Isolation Kit

Get tips on using anti-p62 Protein, C-Terminal Specific Polyclonal Antibody to perform Autophagy assay cell type - MDA-MB-231

Products ARP American Research Products anti-p62 Protein, C-Terminal Specific Polyclonal Antibody

Get tips on using Lipofectamine® 2000 Transfection Reagent to perform DNA transfection Mammalian cells - Primary cells Rat mesangial cells

Products Thermo Fisher Scientific Lipofectamine® 2000 Transfection Reagent

Get tips on using FuGENE® HD Transfection Reagent to perform DNA transfection Mammalian cells - Primary cells Rat mesangial cells

Products Promega FuGENE® HD Transfection Reagent

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