Get tips on using Mouse/Rat Leptin Quantikine ELISA Kit to perform ELISA Mouse - Leptin
Get tips on using Active BDNF (Human, Rat) ELISA Kit to perform ELISA Mouse - GDNF
Get tips on using Rat TGF Beta 1 PicoKine™ ELISA Kit to perform ELISA Rat - TGF-beta 1
Get tips on using Rat Superoxide dismutase [Mn], mitochondrial(SOD2) ELISA kit to perform ELISA Rat - SOD2/Mn-SOD
Get tips on using Rat IL-1 Beta PicoKine™ ELISA Kit to perform ELISA Rat - IL-1 beta
The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.
Get tips on using ScriptSeq Complete Kit (Human/Mouse/Rat) to perform RNA sequencing Mouse - J774
The process of RNA extraction from bacteria, in general, involves an RNA-protective, effective lysis of bacterial cell wall (which may pose difficulties). EDTA promotes loss of outer membrane to provide lysozyme with access to peptidoglycan. Another common method for cell wall lysis is mechanical disruption using a homogenizer (applied for gram-positive bacteria and some strains of gram-negative bacteria). Following lysis, it is necessary to disrupt protein-nucleic acid interactions, which can be achieved by adding sodium dodecyl sulfate (SDS). Next step involves using phenol-chloroform-isoamyl alcohol extraction, where RNA can be obtained from the bottom organic phase, the top phase consists of DNA and the interphase contains proteins. Isoamyl alcohol is an inert and optional addition to this mixture and is added as an anti-foaming reagent to reduce the interphase. Following RNA extraction, the samples should be checked for its quality by gel electrophoresis (23S and 16S rRNAs and 5s rRNA and tRNA bands) or UV spectrophotometric or fluorescence methods.
Get tips on using Active rat PAI-1 functional assay ELISA kit to perform ELISA Rat - Serpin E1/PAI-1
Get tips on using Rat plasminogen activator inhibitor 1,PAI1 ELISA Kit to perform ELISA Rat - Serpin E1/PAI-1
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