The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.
The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.
Get tips on using pSpCas9(BB)-2A-GFP (PX458) to perform CRISPR Human - Repression B3GNT5
Get tips on using pSpCas9(BB)-2A-Puro (PX459) to perform CRISPR Human - Repression GLT25D1
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Get tips on using pSpCas9(BB)-2A-GFP (PX458) to perform CRISPR Human - Deletion AURKB
Get tips on using pSpCas9(BB)-2A-GFP (PX458) to perform CRISPR Human - Deletion GATA1
Get tips on using CMV-CAS9-2A-GFP Plasmid to perform CRISPR Human - Deletion TRIB1
Get tips on using pSpCas9(BB)-2A-Puro (PX459) to perform CRISPR Human - Deletion NOX4
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