ChIP acH3 Goat Rabbit

Get tips on using Anti-TAU antibody produced in rabbit to perform Western blotting Tau

Get tips on using HER2/ErbB2 (29D8) Rabbit mAb #2165 to perform Western blotting HER2

In ChIP, the most vital step is the binding of an antibody and choosing the right antibody. The binding affinity of different types of immunoglobulins to protein A or G differs significantly. Henceforth, it is recommended to choose either protein A or protein G coated beads. If you do not see any product in the positive control, add 5–10 μg of chromatin and 1–5 μg of antibody to each IP reaction and incubate with antibody overnight and an additional 2 hr after adding Protein G/A beads. If no product is observed in the experimental sample, add more DNA to the PCR reaction or increase the number of amplification cycles. Furthermore, if you have any problem with antibodies, make sure to use the ChIP-validated antibody.

Get tips on using Rabbit Anti-Human CHK2 (NT) Affinity Purified pAb to perform Immunohistochemistry chk2 - Rabbit IgG Human -NA-

Get tips on using E-Cadherin (24E10) Rabbit mAb #3195 to perform Immunohistochemistry Human - E-Cadherin

Get tips on using ZO-1 (D7D12) Rabbit mAb #8193 to perform Western blotting ZO-1

Get tips on using P-Cadherin (C13F9) Rabbit mAb #2189 to perform Western blotting P-Cadherin

Get tips on using MMP13 Recombinant Rabbit Monoclonal Antibody (3H13L17) to perform Western blotting MMP-13

Get tips on using Anti-LC3B antibody produced in rabbit to perform Autophagy assay cell type - AR42J

Get tips on using Anti-LC3B antibody produced in rabbit to perform Autophagy assay cell type - NRK