DNA methylation profiling Gene specific profiling MCF-7

Get tips on using EZ DNA Methylation kit to perform DNA methylation profiling Whole genome profiling - mouse T-cell (CD4 / CD8)

Get tips on using EpiTect Bisulfite Kit to perform DNA methylation profiling Gene specific profiling - MG-63 miR-34a

Get tips on using EpiTect MSP Kit to perform DNA methylation profiling Gene specific profiling - Human ovarian tissue MEG3

Get tips on using MethylEasy™ Xceed (ME002) to perform DNA methylation profiling Gene specific profiling - UMR-106 BMP2

Get tips on using EMD Millipore™ Chemicon™ CpGenome™ Universal DNA Modification Kit to perform DNA methylation profiling Gene specific profiling - HepG2 FHIT

Get tips on using EMD Millipore™ Chemicon™ CpGenome™ Universal DNA Modification Kit to perform DNA methylation profiling Gene specific profiling - Hep3B SFRP3

Get tips on using Global DNA Methylation ELISA to perform DNA methylation profiling Whole genome profiling - HCT116, HTC15 human colon cancer cells

Get tips on using EZ-96 DNA Methylation-Gold™ Kit to perform DNA methylation profiling Whole genome profiling - human placenta

Get tips on using MethylEasy™ Xceed (ME002) to perform DNA methylation profiling Gene specific profiling - Rat whole pituitary glands PROP1

Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.