A gross majority of classical apoptotic attributes can be quantitatively examined by flow cytometry, the preferred platform for rapid assessment of multiple cellular attributes at a single-cell level. However, sample preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.
Get tips on using RealTime-Glo™ Annexin V Apoptosis and Necrosis Assay to perform Necrosis LNCaP
Get tips on using RealTime-Glo™ Annexin V Apoptosis and Necrosis Assay to perform Necrosis HUVEC
Get tips on using RealTime-Glo™ Annexin V Apoptosis and Necrosis Assay to perform Necrosis A549
Get tips on using RealTime-Glo™ Annexin V Apoptosis and Necrosis Assay to perform Necrosis HCT 116
Get tips on using CytoSelect™ 24-Well Cell Migration Assay, 8 μm to perform Cell migration / Invasion cell type - BCPAP
Get tips on using CytoSelect™ 24-Well Cell Migration Assay, 8 μm to perform Cell migration / Invasion cell type - SW480
Get tips on using CytoSelect™ 24-Well Cell Migration Assay, 8 μm to perform Cell migration / Invasion cell type - HUVEC
Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase
Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase
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