DNA gel extraction / PCR product purification

Get tips on using FastLane Cell SYBR Green Kit (200) to perform PCR Quantitative real-time PCR - Viral

Get tips on using NucleoSpin® Plasmid to perform DNA isolation / purification Bacteria - Gram negative E.coli

Get tips on using NucleoSpin® Soil to perform DNA isolation / purification Bacteria - Gram positive Lactobacillus

Get tips on using QIAamp DSP Virus Kit to perform DNA isolation / purification Tissue - blood / plasma

Get tips on using EZ1 Virus Mini Kit v2.0 (48) to perform DNA isolation / purification Viral

Get tips on using QIAamp MinElute Virus Spin Kit (50) to perform DNA isolation / purification Viral

Get tips on using DNeasy Blood & Tissue Kit to perform DNA isolation / purification Cells - Primary cells Mouse embryonic fibroblast (MEF)

Get tips on using DNeasy Blood and Tissue Kit (250) to perform DNA isolation / purification Cells - Immortalized cell lines Loucy

I would like to excise a large strand of DNA and insert a new one using CRISPR. My problem is that my strand will be a little over 1kb and I am not sure if this is going to be a limiting factor. Also, how long should the homology arms be for a region of this size?

Get tips on using Gentra Puregene Yeast/Bact. Kit to perform DNA isolation / purification Yeast - Candida parapsilosis