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shRNA gene silencing

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Get tips on using Senescence β-Galactosidase Staining Kit - Cell Signaling to perform Reporter gene assay β-galactosidase substrates - HEK293 human embryonic kidney cells

Products Cell Signaling Technology Senescence β-Galactosidase Staining Kit - Cell Signaling

Get tips on using pMIR-REPORT™ miRNA Expression Reporter Vector System to perform Reporter gene assay luciferase - HEK 293 human embryonic kidney cells

Products Thermo Fisher Scientific pMIR-REPORT™ miRNA Expression Reporter Vector System

Get tips on using β-Galactosidase Enzyme Assay System with Reporter Lysis Buffer to perform Reporter gene assay β-galactosidase substrates - SK-Hep-1

Products Promega β-Galactosidase Enzyme Assay System with Reporter Lysis Buffer

Get tips on using QuikChange II XL Site-Directed Mutagenesis Kit, 10 Rxn to perform Site Directed Mutagenesis (SDM) Human - Deletion K562 c-Myb gene

Products Agilent Technologies QuikChange II XL Site-Directed Mutagenesis Kit, 10 Rxn

Get tips on using EMD Millipore™ Chemicon™ CpGenome™ Universal DNA Modification Kit to perform DNA methylation profiling Gene specific profiling - HepG2 FHIT

Products Fisher Scientific EMD Millipore™ Chemicon™ CpGenome™ Universal DNA Modification Kit

Get tips on using EMD Millipore™ Chemicon™ CpGenome™ Universal DNA Modification Kit to perform DNA methylation profiling Gene specific profiling - Hep3B SFRP3

Products Fisher Scientific EMD Millipore™ Chemicon™ CpGenome™ Universal DNA Modification Kit

Get tips on using EpiTect Bisulfite Kit to perform DNA methylation profiling Gene specific profiling - TCP-1, BCPAP & nthy-ori 3-1 (thyroid tumor cells) METTL7A

Products Qiagen EpiTect Bisulfite Kit

Get tips on using EpiTect Bisulfite Kit to perform DNA methylation profiling Gene specific profiling - TCP-1, BCPAP & nthy-ori 3-1 (thyroid tumor cells) BCPAP

Products Qiagen EpiTect Bisulfite Kit

Get tips on using β-Galactosidase Enzyme Assay System with Reporter Lysis Buffer to perform Reporter gene assay β-galactosidase substrates - human MSCs (mesenchymal stem cells)

Products Promega β-Galactosidase Enzyme Assay System with Reporter Lysis Buffer

Transfection is a powerful technique that enables the study of the function of genes and gene products in cells. Based on the nature of experiments, we may need a stable DNA transfection in cells for persistent gain-of-function or loss-of-function of the target gene. For stable transfection, integration of a DNA vector into the chromosome is crucial which requires selective screening and clonal isolation. By carefully selecting a viral delivery system and related reagents we can ensure safe and highly-efficient delivery of expression constructs for high-level constitutive or inducible expression in any mammalian cell type.

DNA DNA transfection Mammalian cells Immortalized cell lines SH-SY5Y

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