ELISA (kit) Human Serum Cytokine measurements (Multiplex assay) -NA- Human

Get tips on using Human Notch-1 Intracellular Domain Antibody to perform Western blotting Notch1

Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.

Get tips on using Human/Mouse Active Caspase-3 Antibody to perform Western blotting Caspase-3

Get tips on using PE Mouse Anti-Human CD61 Clone VI-PL2 to perform Flow cytometry Anti-bodies Human - CD61

Get tips on using PE Mouse Anti-Human CD26 Clone M-A261 to perform Flow cytometry Anti-bodies Human - CD26

Get tips on using PE-Cy™7 Mouse Anti-Human CD10 to perform Flow cytometry Anti-bodies Human - CD10

Get tips on using PE-Cy™7 Mouse Anti-Human CD56 to perform Flow cytometry Anti-bodies Human - CD56

Get tips on using PE-Cy™7 Mouse Anti-Human CD117 to perform Flow cytometry Anti-bodies Human - CD117

Get tips on using PE-Cy™7 Mouse Anti-Human CD28 to perform Flow cytometry Anti-bodies Human - CD28

Get tips on using PE Mouse Anti-Human CD30 Clone Ber-H83 to perform Flow cytometry Anti-bodies Human - CD30