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2 years ago
2 years ago by Jesualdo Hernández
I am planning to do HIF nuclear extraction using mouse brain tissue. How fast should I be harvesting the tissue before using it for the most optimal results?
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|- Proteins are extracted with a Qiagen AllPrep DNA/RNA/Protein mini kit. Tissues are homogenized following the manufacturer’s instructions with two modifications: (i) addition of protease inhibitors to the lysis buffer (RLT) and (ii) the re-suspension of protein pellets in Modified Reswell Solution.|