Wound healing assay cell type human

miRNA is the inherent gene silencing machinery which can have more than one mRNA target, whereas siRNA can be designed to target a particular mRNA target. By design, both siRNA and miRNA are 20-25 nucleotides in length. The target sequence for siRNAs is usually located within the open reading frame, between 50 and 100 nucleotides downstream of the start codon. There are two ways in which cells can be transfected with desired RNAi: 1. Direct transfection (with calcium phosphate co-precipitation or cationic lipid mediated transfection using lipofectamine or oligofectamine), and 2. Making RNAi lentiviral constructs (followed by transformation and transduction). Lentiviral constructs are time consuming, but provide a more permanent expression of RNAi in the cells, and consistent gene silencing. Direct transfection of oligonucleotides provides temporary genetic suppression. Traditional methods like calcium phosphate co-precipitation have challenges like low efficiency, poor reproducibility and cell toxicity. Whereas, cationic lipid-based transfection reagents are able to overcome these challenges, along with applicability to a large variety of eukaryotic cell lines. When using oligos, the ideal concentration lies between 10-50nM for effective transfection.

Get tips on using Click-iT™ EdU Alexa Fluor™ 555 Imaging Kit to perform Cell cytotoxicity / Proliferation assay cell type - PC-3

Get tips on using Anti-SQSTM1 / p62 antibody (ab56416) to perform Autophagy assay cell type - MEFs (mouse embryonic fibroblasts)

Get tips on using Atg12 Antibody (Mouse Specific) #2011 to perform Autophagy assay cell type - MEFs (mouse embryonic fibroblasts)

Get tips on using Annexin V-FITC Apoptosis Kit to perform Apoptosis assay cell type - Sao2-2, MG-62

Get tips on using CYTO-ID® Autophagy detection kit to perform Autophagy assay cell type - MDA-MB-231

Get tips on using Anti-LC3B antibody produced in rabbit to perform Autophagy assay cell type - Mouse lung tissue

Get tips on using Anti-LC3B antibody produced in rabbit to perform Autophagy assay cell type - Mouse embryonic fibroblasts

Get tips on using CYTO-ID® Autophagy detection kit to perform Autophagy assay cell type - Mouse embryonic fibroblasts