3D Cell Culture Media Human breast cancer MCF-7 cells-Mammospheres

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Found 3 matching solutions for this experiment

DMEM/F-12, no phenol red

Thermo Fisher Scientific

Protocol tips
Seed 3000 cells/well (MCF7)
Use of pHEMA (Sigma) -coated 6-well plates
Score of mammospheres > 50μm
MammoCult™ Human Medium Kit

STEMCELL technologies

Protocol tips
Seed at a density of 5,000 cells/mL
Use 6-well ultra-low-attachment plates
Count mammospheres ≥50 μm in diameter
Protocol tips
Seed of cells at several densities (10, 50, 100, 1000 cells per well)
Use of 96-well ultra-low attachment plates
Count of >50μm mammospheres
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