Get tips on using APC anti-human/mouse CD49f Antibody to perform Flow cytometry Anti-bodies Human - CD49f/ITGA6
Get tips on using Recombinant Anti-Integrin beta 1 antibody [EPR16895] (ab179471) to perform Western blotting ITGB1
Get tips on using Phusion Site-Directed Mutagenesis Kit to perform Site Directed Mutagenesis (SDM) Human - Point mutation A549 ISG15
Western blotting is a widely used technique to size separate proteins from a pool of cell or tissue lysates. The technique has 4 major steps: a) gel electrophoresis, b) blocking and treatment with antigen specific antibody, c) treatment with secondary antibody and finally d) detection and visualization. Though western blotting is a widely used technique, detection of specific proteins depends on several factors, the major ones are antibody concentration, incubation time and washing steps. Key points for obtaining clean blots are: always prepare fresh buffer solutions and optimize antibody concentration. Given the advent of high-throughput protein analysis and a push to limit the use of lab consumables, onestep antibodies are developed which recognise protein of interest and also contain a detection label.
Western blotting is a widely used technique to size separate proteins from a pool of cell or tissue lysates. The technique has 4 major steps: a) gel electrophoresis, b) blocking and treatment with antigen specific antibody, c) treatment with secondary antibody and finally d) detection and visualization. Though western blotting is a widely used technique, detection of specific proteins depends on several factors, the major ones are antibody concentration, incubation time and washing steps. Key points for obtaining clean blots are: always prepare fresh buffer solutions and optimize antibody concentration. Given the advent of high-throughput protein analysis and a push to limit the use of lab consumables, onestep antibodies are developed which recognise protein of interest and also contain a detection label.
Get tips on using Q5® Site-Directed Mutagenesis Kit to perform Site Directed Mutagenesis (SDM) Human - Point mutation Huh7 ISG20
Get tips on using KAPA Stranded RNA-Seq Kits with RiboErase (HMR) to perform RNA sequencing Rat - Lung tissue
Get tips on using KAPA Stranded RNA-Seq Kits with RiboErase (HMR) to perform RNA sequencing Human - MCF-7
Get tips on using KAPA Stranded RNA-Seq Kits with RiboErase (HMR) to perform RNA sequencing Human - MDA-MB-231
Get tips on using QIAprep Spin Miniprep Kit to perform Plasmid Isolation Salmonella enterica serovar Indiana (S. Indiana)
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