Mammalian cell culture media

Get tips on using LIVE/DEAD™ Fixable Near-IR Dead Cell Stain Kit, for 633 or 635 nm excitation to perform Live / Dead assay mammalian cells - rat testicular tissue

Get tips on using CultreCoat Medium BME Cell Invasion Assay, 96 well to perform Cell migration / Invasion cell type - HCT116

Get tips on using CultreCoat Medium BME Cell Invasion Assay, 96 well to perform Cell migration / Invasion cell type - Skov3

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Get tips on using Iscove’s Modified Dulbecco’s Medium to perform Stem cell Differentiation media iPSCs or hESCs differentiation into cerebellar neuroepithelium (NE)

Get tips on using Gibco™Essential 8™ Medium to perform Stem cell Differentiation media hPSC or hiPSCS differentiation into myogenic cells

Get tips on using Remel™ Candida albicans Test Kit to perform Cell Culture Contamination Detection Kit Yeast

Get tips on using AMPIPROBE® Candida 1 Assay Kit to perform Cell Culture Contamination Detection Kit Yeast

Get tips on using VetMAX™ West Nile Virus Kit to perform Cell Culture Contamination Detection Kit Virus