siRNA / miRNA gene silencing Rat Schwann cells

DNA-protein interactions are studied by using ChIP. The basic steps in this technique are crosslinking, sonication, immunoprecipitation, and analysis of the immunoprecipitated DNA. During ChIP, if chromatin is under-fragmented or fragments are too large which can lead to the increased background and lower resolution. Shorter cross-linking times (5-10 min) and/or lower formaldehyde concentrations (<1%) may improve shearing efficiency. If Chromatin is over-fragmented, then optimize shearing conditions for each cell type to improve ChIP efficiency. Over-sonication of chromatin may disrupt chromatin integrity and denature antibody epitopes. If you do not see any product or very little product in the input PCR reactions, add 5–10 μg chromatin per IP.

Get tips on using NeuroCult™ Basal Medium (Mouse & Rat) to perform 3D Cell Culture Media Mouse embryonic neurospheres

Get tips on using Absolutely RNA miRNA Kit to perform RNA isolation / purification Cells - immortalized HT-29

Get tips on using Active rat PAI-1 functional assay ELISA kit to perform ELISA Rat - Serpin E1/PAI-1

Get tips on using Rat plasminogen activator inhibitor 1,PAI1 ELISA Kit to perform ELISA Rat - Serpin E1/PAI-1

Get tips on using miRcute miRNA Isolation Kit to perform RNA isolation / purification Cells - immortalized MDA-MB-157

Get tips on using ANGPTL3 (mouse/rat) Dual ELISA Kit to perform ELISA Mouse - Angiopoietin-Like 3 (AngptL3)

Get tips on using Beta-Glo® Assay System to perform Reporter gene assay β-galactosidase substrates - rat mesenchymal stem cells (MSCs)

Get tips on using Purified Rat Anti-Mouse IFN-γ to perform Flow cytometry Anti-bodies Mouse - IFN-γ

Get tips on using APC Rat Anti-Mouse IFN-γ to perform Flow cytometry Anti-bodies Mouse - IFN-γ