Protein expression and purification Insect cells Hi5

Get tips on using Qproteome Bacterial Protein Prep Kit to perform Protein isolation Bacteria - Salmonella enterica

Get tips on using Qproteome Bacterial Protein Prep Kit to perform Protein isolation Bacteria - Pseudomonas aeruginosa

Get tips on using Qproteome Bacterial Protein Prep Kit to perform Protein isolation Bacteria - Bacillus subtilis

Get tips on using Qproteome Bacterial Protein Prep Kit to perform Protein isolation Bacteria - Staphylococcus aureus

Get tips on using Qproteome Bacterial Protein Prep Kit to perform Protein isolation Bacteria - Salmonella paratyphi A

Get tips on using Qproteome Mammalian Protein Prep Kit to perform Protein isolation Tissue - Human tissue C-MFPE samples

Get tips on using miRCURY RNA Isolation Kit to perform RNA isolation / purification Cells - immortalized SKBR3, MDA-MB231 and MCF7

Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Cells - immortalized SKBR3, MDA-MB231 and MCF7

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.