rna-isolation-purification-tissue-rat-spinal-cord

Get tips on using Aurum™ Total RNA Mini Kit to perform RNA isolation / purification Cells - primary mouse cortical neurons

Hello! I used Trizol to extract total RNA from in-vitro cultured bacteria (1 X 10^8 cells). After phase separation, I mixed ~0.4 ml of the upper phase which contains RNA with 0.5 mL cold isopropanol. However, the amount of RNA when measured in Nanodrop was very low. In addition, the ratio between 260 and 230 was around 0.1 to 0.5. Is there a chance that my sample was contaminated by the Trizol reagent? When I collected the aqueous phase I made sure to not touch the lower phase. What should I do?

Get tips on using TIANamp Genomic DNA Kit to perform DNA isolation / purification Tissue - bone

Get tips on using QIAamp DNA Mini Kit to perform DNA isolation / purification Tissue - bone

Get tips on using QIAamp DNA Micro Kit to perform DNA isolation / purification Tissue - adipose

Get tips on using NucleoSpin® RNA to perform RNA isolation / purification Bacteria - Gram negative Bordetella pertussis

Get tips on using NucleoSpin® RNA to perform RNA isolation / purification Cells - immortalized MIA PaCa-2

Get tips on using REALTOTAL RNA Spin Plus to perform RNA isolation / purification Cells - primary human aortic smooth muscle cells

Get tips on using Direct-zol RNA Kits to perform RNA isolation / purification Cells - immortalized PC-3