siRNA / miRNA gene silencing Human UCC (urothelial cancer cell lines)

Get tips on using EpiTect Bisulfite Kit to perform DNA methylation profiling Whole genome profiling - OVCAR-3 human ovarian cancer

RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.

Get tips on using Gentra Puregene Cell Kit Plus (6.7 x 109) to perform DNA isolation / purification Cells - Immortalized cell lines H1 hESc

Get tips on using QIAamp UCP DNA Micro Kit to perform DNA isolation / purification Cells - Immortalized cell lines MKN45

ROS has a very short half-lives in biological environment as they are influenced by exposure to ambient oxygen. As it is highly reactive and hard to measure care should be taken to ensure the stability of the sample during isolation, preparation, storage, and analysis.

Get tips on using MACSprep™ HLA B/T Cell Isolation Kit, human to perform Cell Isolation HLA B/T

Wound healing assay can be challenging due to inconsistencies and variations while making a wound on the confluent cell monolayer, consequently leads to wounds of varying sizes and widths. Moreover, this assay causes damage to the cells that are at the edge of the wound, which can prevent cell migration into the wound site and healing. The best solution is to use the standard wound healing assay kits using either combs or inserts to make a defined wound field or gap and prevent the well-to-well variation in these assays.

Get tips on using hMSC Human Mesenchymal Stem Cell Chondrogenic Differentiation Basal Medium to perform Stem cell Differentiation media mPericytes differentiation into Chondrogenic cells

Get tips on using AmpFLSTR™ Identifiler™ PCR Amplification Kit to perform Cell line authentication Ovarian cancer cell line PA-1

Get tips on using AmpFLSTR™ Identifiler™ PCR Amplification Kit to perform Cell line authentication Ovarian cancer cell line ES-2