Get tips on using Monoclonal Anti-Proliferating Cell Nuclear Antigen antibody produced in mouse to perform Western blotting PCNA
Get tips on using PerCP-Cy™5.5 Mouse Anti-Human HLA-DR to perform Flow cytometry Anti-bodies Human - HLA-DR
Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.
The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.
Get tips on using MLM3636 to perform CRISPR Human - Activation VEGFA
Get tips on using lentiCRISPR v2 to perform CRISPR Human - Repression
Get tips on using Purified Mouse Anti-Human MSH-2 Clone G219-1129 (RUO) to perform Immunohistochemistry Human - MSH2
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